Polytene chromosome aberrations based genotoxicity appraisal by implementing insect genome

Abstract

Present research work deals with evaluation of clastogenic and cytotoxic effects of dichlorvos using salivary gland polytene chromosomes. Exposure of pesticide was given Drosophila melanogaster larvae which were sacrificed for temporary squash preparation of polytene chromosome, along with controls. The structural aberration in treated stocks were incidences of ectopic pairing, inversion, breaks, chromosomal fusion, asynapsis and translocation with respective number 40, 23, 15, 12, 10 and 8 in treated stock while in control stock main aberrations are, inversion chromosomal fusion and ectopic pairing with respective value 32, 15 and 9 . Subsequently, statistical analysis was done by applying student ‟t” test and it was concluded that dichlorvos induced statistical significant genotoxicity in exposed larvae in comparison to control.