Cisplatin induced histopathological changes in the liver and its amelioration by Andrographis paniculata

Abstract

The role of ethanolic extract of Andrographis paniculata [AP], in preventing cisplatin [CP] induced histopathological changes in rat hepatic tissue was studied. Sixty Wistar Albino rats were divided into five groups with twelve rats in each group. Group I served as normal control group. To group II rats, CP was administered at 7.5 mg/kg body weight intraperitoneally for single dose. Rats in group III served as AP control and were administered AP at the dose of 500 mg/kg body weight for 45 days. Group IV rats were pre-treated with AP fifteen days prior to CP administration and followed by AP treatment for 45 days. Rats in group V were administered with CP and concurrently treated with AP extract at 500 mg/kg by oral gavaging for 45 days. The liver samples collected at 7th, 14th, 28th, and 45th day of the study were subjected for histopathological studies. Results documented that, CP produced severe congestion and dilation of central vein, portal vessels, sinusoids congestion and dilation, presence of necrotic and apoptotic cells and polymorphonuclear cell infiltration in the liver. The AP treatment groups revealed improvement in the liver architecture, while pretreatment of AP produced the much earlier amelioration of the pathological changes. The study indicated that, prior administration of AP inhibited the CP injury and has good prophylactic effect.