Lignans from of the seeds of Brochoneura acuminate (Myristicaceae) and the antioxidant and wound healing properties of the methanol extract

Abstract

The seeds of Brochoneura acuminata (Lam.) Warb. (MYRISTICACEAE), endemic to Madagascar, are used in Malagasy traditional medicine for their wound healing virtue in the South-Eastern part of the island. Two lignans named Otobanone (1) and 1-hydroxyotobain (2) were isolated for the first time from the methanol extract. Their chemical structures were established using MS and NMR technics. The free radical DPPH scavenging assay was used to determine the antioxidant capacity of the methanol extract. The wound healing effect of the methanol extract was assessed on the rat dorsal created experimental wound. The methanol extract showed a potent scavenging capacity with an IC50 value of 105.31 ± 0.24 μg / mL. Topical application of both 2% (w/w) methanol extract of the seeds of B. acuminata and OFM® ointments in excision wound model significantly increased (p