Development and validation of methodology for estimation of fenpropathrin and pyriproxyfen in chilli and soil through GC-ECD and NPD

Abstract

The objective of the study is to standardize methodology for estimation of fenpropathrin and pyriproxyfen in chilli and soil. QuEChERS technique is used for estimation of pyriproxyfen and fenpropathrin conducted through GLC equipped with NPD and ECD in chilli and soil. “Retention time” for pyriproxyfen and fenpropathrin was observed to be 4.10 and 4.73 min. LOQ and LOD of pyriproxyfen and fenpropathrin was quantified to be 0.01 mg kg-1, 0.05 mg kg-1 and 0.003 mg kg‑1, 0.02 mg kg‑1. Mean recoveries of pyriproxyfen in chilli and soil samples spiked of 0.01, 0.05 and 0.10 mg /kg, range about 80.97 to 88.33 per cent and found to be more than 80 per cent whereas for fenpropathrin in chilli and soil samples spiked with 0.05, 0.25 and 0.50 mg/ kg levels ranged from 80.03 to 89.51 per cent. RSDr for pyriproxyfen in chilli and soil at 0.01 - 0.50 mg/ kg ranged over 2.49 to 8.65, 2.73 to 10.13 per cent whereas for fenpropathrin in chilli and soil at 0.05-0.50 mg/ kg at 4.79 to 6.26, 2.90 to 6.95 per cent. The between-batch recoveries and reproducibility for pyriproxyfen and fenpropathrin in chilli and soil were examined at 0.01mg kg-1and 0.05mg/ kg. The reproducibility of pyriproxyfen and fenpropathrin in distinctive substrates ranged from about 6.15 to 10.48 per cent and all measurements are within 15 per cent at all concentrations.