Polymorphism study of Fec X gene in indigenous sheep of Assam

Abstract

The present study was conducted to check the polymorphism of Fec X gene in the indigenous sheep breeds of Assam. A total of 50 unrelated sheep covering Barpeta, Bongaigaon, Goalpara, Hekera and Kamrup districts of Assam was taken into consideration for the research work. Blood samples were collected and genomic DNA was isolated. PCR amplification of the DNA samples produced 141 bp amplified product. The PCR-RFLP technique was employed using Hinf1 restriction enzyme which resulted into a single type of banding pattern with two fragments of 110 and 31 bp in all the samples studied. Fec X gene was found to be monomorphic in the studied population.