Sequence characterization of amplifying region by using RAPD marker for bottle gourd (Lagenaria siceraria)

Abstract

Bottle gourd [Lagenaria siceraria (Mol.) Standl.] (2n = 2x = 22), also known as opo squash or long melon, is diploid belonging to the genus Lagenaria of the Cucurbitaceae family with a genome sizea of ~334 Mb. Bottle gourd comes under in different sizes some are long, some are round and some are dumbbell shaped. DNA was isolated from 4 varieties of Lagenaria (2 long Narendra Shivani and Narendra Rashmi & 2 Round Narendra Madhuri and Narendra Prabha) and was done with 32 primers. RAPD was done in 4 varieties of Lagenaria (2 long Narendra Shivani and Narendra Rashmi & 2 Round Narendra Madhuri and Narendra Prabha) and was done with 32 primers. Out of 32 primers, 8 primers shown good amplification and dendrogram was generated using NTSYS analysis. Relationship of Lagenaria was established as shown by dendrogram. Narendra Rashmi and Narendra Shivani (both long) falls in one group as shown in dendrogram. 1200 bp long variety specific DNA was identified with OPK 17 primer. 1200 bp band in Narendra Shivani was more intense in Narendra Shivani than Narendra Rashmi. Longest nature of Narendra Shivani may be due to repeat copy of this DNA as amplican was more intense in Narendra Shivani then Narendra Rashmi.