Detection of infectious bursal disease virus in tissue samples by immunoperoxidase staining technique

Abstract

Chicken is the only host that manifests clinical signs and its pathogenicity is correlated with the distribution of antigens in organs. IBDV caused lymphoid depletion which can be detected by Immunohistochemistry. In this study, Immunohistochemistry was used to detect the viral antigen in frozen sections. A hyper-immune serum produced in rabbits through injection of IBD vaccine and used as a primary antibody in present study. Positive IBDV antigen staining of lymphoid cell population in bursa of Fabricius, Spleen Caecal tonsils, Thymus and Muscles was observed by using indirect Immunohistochemtry. The total number of sample in this study was 183 and out of which 165 showed positive characteristic golden brownish colour.