Detection and molecular characterization of Bean Common mosaic virus in mungbean

Abstract

Bean common mosaic virus (BCMV) is an important seed borne pathogen. The virus caused downward leaf rolling, thickening of leaves, mosaic, leaf deformation, necrosis of tissues of abaxial side of leaves, necrosis of apical stem portion. Vigna mungo, Punicum granatum, Vigna unguiculata and Ricinis communis gave positive reaction to BCMV antisera and potyvirus antisera. The infected leaves of IR-16, Meha and K-851 gave strong positive reaction with BCMV specific antisera with O.D. value of 3.99, 3.91 and 3.37, respectively as compared to positive control with OD value of 2.20.Transmission electron microscope studies revealed the presence of BCMV as flexuous particles of 823 nm long.The RT-PCR amplified product when run on 1 per cent agarose revealed the presence of predicted ~1300 bp product.