Recovery of oocytes from ovarian follicles by aspiration method in buffaloes

Abstract

In the present study total of 155 ovaries were procured from a local slaughter house and follicular size categorization based on their diameter using vernier caliper as 5 mm size was carried out. Aspiration technique of oocytes retrival from buffalo ovarian follicles was done using an 18 gauge needle attached to a 5 ml syringe. Follicular contents were poured in a searching dish which had a square grid pattern and Cumulus Oocyte Complexes (COCs) were searched under a stereozoom microscope. The overall percentage of small, medium and large follicles in the ovaries were recorded as 16.29 ± 0.94%, 8.14±0.60%, 5.35 ± 0.76%, respectively. The overall recovery rate of COCs was 38%. The percentage of these oocytes were 16.74% (A), 15.25% (B), 25.26% (C), 18.33% (D) and 29.87% (E) respectively. Aspiration method is a quick method of oocytes recovery.