Genetic diversity analysis of CGMS lines A, B and restorer lines in tetraploid cotton (Gossypium hirsutum L.) using RAPD markers

Abstract

Random amplified polymorphic DNA (RAPD) analysis was employed in 5 CGMS lines, 5 B-lines and 8 restorer lines of tetraploid cotton (Gossypium hirsutum). The 20 primers selected generated 572 amplification products. Statistical analysis was carried out using NTSYS- pc software and a dendrogram was generated using Jaccard’s similarity coefficients. The values for similarity coefficients ranged from 0.05 to 0.99. The cluster tree analysis showed the grouping of 18 genotypes in two major A and B groups at similarity coefficient of 0.35. The major group A consists of twelve genotypes and group B consists of remaining six genotypes. Cluster analysis showed clear-cut separation of the CGMS lines, B-lines and restorer lines. The primer OPO - 1 with 4 unique bands could distinguish K34007A and K34007B line and similarly Jhorar A and Jhorar B line. The primer OPO - 11 could distinguish restorer lines CIR 28 and CIR 38 scoring 4 and 3 unique bands respectively. The primers OPO - 12 and 20 also distinguished restorer lines CIR 28 and CIR 38. The primers OPO - 15 and 16 could distinguish restorer lines CIR 26 scoring 1 and 2 unique bands respectively.