Outer membrane proteins (OMPs) profiling of a field isolate of Salmonella Typhimurium: Isolation and its characterization

Abstract

Salmonella Typhimurium is a significant zoonotic pathogen affecting human and animal health. In the present study, a S. Typhimurium isolate, previously isolated from poultry samples was revived and re-confirmed as S. Typhimurium by isolation in Hektoen enteric agar followed by molecular detection. PCR with the stn gene, his J gene and Typh gene primers revealed the specific amplicon of size 617 bp, 496 bp and 402 bp respectively. Antibiotic sensitivity pattern of the isolate was also determined. Isolate was then cultured in LB broth in bulk and outer membrane proteins (OMPs) were isolated by ultracentrifugation. The OMPs were successfully isolated and confirmed by SDS-PAGE analysis. The band pattern revealed nine major bands ranging from 11-90 kDa.