Identification of polymorphism in ABCG2 gene in Rathi cattle

Abstract

In present study, Rathi cattle were selected to analyze ABCG2 genes for polymorphism. Total 160 lactating animals of Rathi cattle from livestock research station (LRS), Nohar and Bikaner were selected. Blood samples were collected and DNA isolated by kit method. For Exon 7-partial intronic region, Exon 14 and Intron 15 region of ABCG2 gene primers were constructed based on available sequences of ABCG2 gene in the NCBI GenBank database. Fragments of 393, 207 and 323-bp of exon 7-partial intronic region, exon 14 and intron 15 region of ABCG2 gene, respectively were amplified by a polymerase chain reaction in a final reaction volume of 25μl. The PCR-RFLP and PCR-SSCP method was used according to region of ABCG2 gene. In exon 7-partial intronic region and exon 14 of ABCG2 gene shows monomorphism. In intron 15 region of ABCG2 gene two SSCP patterns were observed and it shows polymorphic nature of this region. In intron 15 region of ABCG2 gene observed two alleles and two genotypes with frequency 0.85, 0.15, 0.70 and 0.30, respectively, were observed. In present study, two regions of ABCG2 gene observed monomorphic nature and one region, intron 15 region of ABCG2 gene revealed polymorphic nature.