Preliminary quantification of Phytochemicals in methanolic extract of Roylea cinerea (D. Don) Baillon by using HPTLC technique

Abstract

Roylea cinerea is a medicinal plant of monotypic genus belongs to family Lamiaceae (Incl. Verbenaceae) family, ranged from Kashmir (India) to Nepal in Western Himalayas. The present study reviews about macroscopical-botanical description for authenticity of selected plant with macroscopical-physical parameters like colour, odour, leaf shape-margins-venation etc. were observed for the identification of Roylea cinerea. It was important to authenticate taken plant before its methanolic extraction for preliminary quantification of chemicals constituents. In Phytochemical analysis, constituents are quantified by visualizing TLC plate under UV light at different wavelength i.e., 254 nm, 366 nm and 566 nm to get chromatograph of HPTLC which showed different Rf for different phytochemicals present in methanolic extract of plant.