In vitro assessment of quantitative analysis of phytochemicals, iron (II) chelating activity and antimicrobial efficacy of methanolic solvent extract of citrus maxima leaf

Abstract

Herein, we report the quantitative analysis of phytochemicals, iron (II) chelating activity, and antimicrobial efficacy of Citrus maxima (Pomelo) leaves in methanol extract using Folin-Ciocalteu’s colorimetric assay, aluminium chloride colorimetric assay, Ferrous-ferrozine complex inhibition assay and agar disc diffusion method. The total phenol contents extracted as 117.68±0.63 mg of GAE/g of dry extract and 14.39±0.09 mg of CE/g of dry extract and for total flavonoid it was 28.55±0.39598 mg of CE/g of dry extract. The inhibitory concentration (IC50) of ferrous ion chelating activity was recorded as 323.79 μg/mL. Also, this chelating activity increases with the extract concentrations. On the other hand, this leaf extracts displaced a concentration dependent inhibition of microorganisms, the eleven different cultures responded to samples, by virtue of which we obtained in the range of 5.5-12 mm zone of inhibition. Especially, this leaf extract shows a satisfactory antibacterial efficacy on Salmonella paratyphi, Salmonella typhi and V. cholerae C6706. The outcomes of the study corroborate the extract samples, and can even be a brand-new source for several drug discoveries.