SDS-PAGE analysis of urinary peptides in indigenous cow

Abstract

Urinary proteins and peptides are biologically active molecules with diverse structural and antimicrobial properties for combating infections and microbial drug resistance. The aim of this study was to identify the proteins excreted in cow urine. In the present study, fresh urine samples were collected from ten healthy cycling indigenous cows and urinary proteins and peptides were extracted using ion exchange chromatography. Extracted urinary proteins and peptides of healthy cow were analyzed by a combination of SDS-polyacrylamide gel electrophoresis and coomassie brilliant blue stain. We have been able to identify multiple protein bands from cow urine with a molecular weight ranging from 22-153kDa.