The increase of survival rate of shrimp Penaeus monodon after administrated with VP28

Abstract

White spot Baculovirus (WSBV) infection of shrimp is characterized by high mortality of tiger shrimp Penaeus monodon. The gross signs of moribund shrimp are present of white spot, initially circular, inside cuticle, sometimes accompanied by overall red body coloration. The 5 major protein were isolated from WSBV are 15 kDa (VP15), 19 kDa (VP19), 24 kDa (VP24), 26 kDa (VP26) and 28 kDa (VP28). The isolated protein were then purified and characterized to be VP19, VP24, and VP28. Purified protein injected to shrimp to detect whether they have a protective immunity against WSBV infection. The result showed protection of infected shrimp against WSBV after administration of VP19, VP24 and VP28 are 20%, 0% and 60%, respectively. VP28 injected shrimp have a high production of phenoloxidase 24.1822 U/min/mg and after WSBV challenged 44.6067 U/min/mg. Based on the result suggest that VP28 of WSBV is potentially to increase immune response in penaeid shrimp.