In vitro bioefficacy of bioagents against pathogenic mycoflora of linseed seeds

Abstract

Linseed (Linum usitatissimum L.), the most grown oilseed crop is suffered by several diseases causing pathogenic organism, most of which are seed borne, causing significant quantitative and qualitative losses. Though, majority of seed borne fungi are being managed with fungicidal seed treatments, but due to their harmful effects, their use needs to be reduced. Therefore, present research work entitled “in vitro bioefficacy of bioagents against pathogenic mycoflora of linseed seeds” was conducted to asses bioefficacy of bioagents against three major pathogenic seedborne fungi viz., Fusarium oxysporum f. sp. lini, Alternaria lini, and Curvularia lunata, by applying Dual culture technique. The results revealed that all of the seven test bioagents significantly inhibited mycelial growth of Fusarium oxysporum f. sp. lini, Alternaria lini, and Curvularia lunata over untreated control. However, in case of Fusarium oxysporum f. sp. lini, the most effective bioagent was T. harzianum (86.85%) followed by T. hamatum (85.48%), A. niger (83.15%), T. koningii (81.60%) while P. fluorescens (52.04%) was least effective. Whereas, for Alternaria lini, the most effective bioagent was T. hamatum (91.60%) followed by A. niger (85.77%), T. harzianum (82.44%), T. asperellum (80.52%) which caused highest mycelial growth inhibition while least inhibition was caused by P. fluorescens (57.60%). Whereas, for Curvularia lunata bioagent T. harzianum (88.15%) followed by T. hamatum (85.07%), T. lignorum (82.60%) caused highest mycelial growth inhibition while least inhibition was with P. fluorescens (54.26%).