Molecular detection of canine parvovirus from haemorrhagic enteric affections of dog in Orathanadu region, Tamil Nadu, South India

Abstract

The present study deals with the rapid detection of Canine Parvo Viral (CPV) infection in various breed of dogs of Cauvery Delta Region, Tamilnadu by molecular means of diagnosis employing the polymerase chain reaction (PCR) method. A total of 168 haemorrhagic fecal samples were collected and out of those 112 were found positive by PCR. Among different age groups, 154/80,55/67 and 3/21 dogs were positive in pups aged 0-3 weeks,4–8 and 9–12 weeks respectively. All positive samples were from unvaccinated dogs. The breed of Labrador was found to be the most susceptible breed (n = 43) to Parvo viral infection. The positivity of confirmation (112/168: 66%) concludes PCR being a reliable one for early diagnosis and the study on variants of CPV-2b was ascertained by PCR and being the first report on molecular detection of CPV infection in dogs in this region. Type CPV-2c was not detected among the examined samples. On review of literature and on observance of recent reports the CPV Variant (CPV-2b) in India is gaining importance and mortality of pups dure to CPV infection is high and precise and early diagnosis of CPV infection warrants early intervention of prior vaccination in all breeds of dogs against the deadly viral infection.