Isolation and identification of Triazole group fungicide degrading isolates from grape rhizosphere of major grape growing districts of Maharashtra, India

Abstract

Grape (Vitis vinifera L.) is the most important temperate fruit crop that has acclimatized to the subtropical and tropical agro-climatic conditions. Thirty soil samples were collected from grape vineyards rhizosphere of different locations of Nashik, Ahmednagar, Pune, Solapur and Sangli districts of Maharashtra during 2017-18. The 17 isolates (FDB1 to FDB17) were obtained from soil samples which had ability to degrade difenoconazole, myclobutanil and fluopyram+ tebuconazole fungicides. On the basis of colony characteristics as well as biochemical test, eight Gram +ve strains and nine Gram -ve strains were identified up to the generic level. The Gram +ve strains were 3 strains of Micrococcus spp., 2 strains of Bacillus spp., a strain of Staphylococcus spp., Inquilinus spp. and Lysinibacillus spp. In Gram –ve strains viz., eight strains of Pseudomonas spp. and a strain of Stenotrophomonas spp. were identified which has ability to use sole carbon source from fungicide for its growth.