Effect of melatonin supplementation on heat shock proteins expression profile in buffalo calves under summer stress

Abstract

A study was undertaken to investigate the effect of melatonin supplementation on heat shock proteins (HSPs) (HSP 60, HSP70, HSP 90 and HSP 110) expression profile in buffalo calves under summer stress. Twelve healthy Murrah buffalo male calves of 6 month to 1 year age group were taken for the study. Buffalo calves were divided into control (CG) and treatment (TG) group. In TG, Melatonin (18mg/50 kg BW) was injected subcutaneously, two times at 1st and 20th day. Blood samples were collected on 0th, 7th, 14th, 21st, 28th, 35th and, 42nd days. The relative expression of genes like HSP 60, HSP 70, HSP 90 and HSP 110 was studied in peripheral blood mononuclear cells. Between the groups, the mRNA expression of HSP70, 90 and 110 differed significantly (P