In vitro antimicrobial assay of leaves, bark and fruits of Ficus auriculata collected from two different regions of Uttarakhand

Abstract

The present study is to evaluate the antimicrobial efficacy of Ficus auriculata fruits, leaves and bark collected from two different regions of Uttarakhand i.e, Almora and Haldwani. These parts of plants extracted in hexane, chloroform and methanol by successive soxhlet extraction technique. For antimicrobial assay, activity was done by agar well diffusion method against four human pathogenic bacteria (two gram positive and two gram negative bacteria).The results concluded that Almora leaves hexane extract possessed the higher antibacterial potential on increasing dose dependent manner at 800µg/ml (15.7±0.25 mm) Haldwani fruits hexane extract (15.5±0.25 mm) against E. coli. Almora bark chloroform extract inhibits the bacteria E. coli and B. subtilis with maximum zone (14±0.11 mm) and (12.4±0.15 mm). Haldwani leaves methanol extract showed the maximum inhibition zone(13±0.57 mm) against E. coli and Haldwani bark methanol extract showed the maximum inhibition zone (12.9±0.057 mm). These naturally occurring antimicrobials agents due to its safe and non toxic nature.