The effect of cold preculture, media and incubation temperatures on callus induction via Antherculture in Brinjal (Solanum melongena L.)

Abstract

The study was conducted to investigate the effect of pre-treatments, incubation temperature and culture media in different eggplant genotypes through Anther culture. The eggplants were given with 3 different hours of pre-treatments in combination with two different incubation temperatures (25 oC for 3 weeks and 35 oC for 8 days under dark condition). One cross (IGB-17 X Kasi Taru) was implanted in C medium supplemented with different hormones. Among different treatment combinations the treatment C5 media +3 hours pre-treatment + incubation at 25℃ for 3weeks showed maximum embryogenic calli induction with response frequency 74.05 per cent and took least number of days for callus initiation (10 days) fallowed by C2+2h+25 ℃ had % callus induction frequency with minimum days of 10.71, respectively.