Cryopreservation of Indigenous bull semen (Zobawng) of Mizoram: Effect of fructose on extender

Abstract

A total of 36 ejaculates were collected from three indigenous bulls (Zobawng) of Mizoram. The semen samples were evaluated to find out the effects of fructose i.e. Tris egg yolk citric acid fructose glycerol (TEYCAFG) and Egg yolk sodium citrate glycerol (EYSCG) extenders on freezability of indigenous bulls’ (Zobawng) semen of Mizoram in terms of sperm motility, per cent live sperm, HOSST reacted spermatozoa and acrosomal integrity. The mean sperm motility, per cent live sperm, HOSST and acrosomal integrity in TEYCAFG extender at pre and post freezing were (69.53±0.31 and 45.36±0.59%), (76.36±0.35 and 53.03±0.45%), (67.91±0.45 and 44.92±0.56%) and (75.17±0.58 and 63.25±0.56%) respectively, and in EYSCG extender were (45.67±0.48 and 33.08±0.36%), (54.56±0.41 and 38.08±0.28%), (47.86±0.44 and 34.61±0.40%) and (57.22±0.31 and 45.89±0.48%) respectively. The mean motility, live sperm, HOSST and acrosomal integrity in both TEYCAFG and EYSCG extenders were significantly (P