Comparative study of different solvents seed extracts of Azadirachta indica (neem) and its in-vitro acaricidal properties against Rhipicephalus (B.) microplus

Abstract

The seeds of Azadirachta indica (Neem) were analyzed for gradient yields in different solvents and its acaricidal properties. The solvents used were aqueous (distilled water), ethanol, hydroethanol and methanol in Soxhlet apparatus for the extraction of extracts. The acaricidal properties of A. indicaseed extract was assessed by using the solution of 8% aqueous, ethanolic, hydro-ethanolic and methanolic extracts. The highest yield obtained was methanolic extract about 14.84%, followed by ethanolic, hydroethanolic and aqueous extract 13.24%, 9.76% and 8.12% respectively. Variable amounts of yields might be possible due to the method, solvents, time and temperature applied for the extraction. During the assessment of acaricidal properties of A. indicaseed extract, aqueous extract was found more effective as compared to ethanolic extract, hydroethanolic extract and methanolic extract and lowest efficacy was recorded in methanolic extract, might be happened by the presence of sufficient biomolecules in aqueous extract.