Evaluation of antioxidant profile in subclinical mastitis in dairy buffaloes

Abstract

The present research investigation was taken up to find out level of oxidative stress in dairy buffaloes suffering from subclinical mastitis with emphasis on Staphylococcus aureus infection at Pantnagar, Uttarakhand, India. Buffaloes were screened for subclinical mastitis (SCM) based on physical examination of udder, California mastitis test (CMT), Somatic cell count (SCC) and Differential cell count (DCC) on quarter’s milk samples. Intramammary infection (IMI) was confirmed by cultural examination of milk. A total of 30 buffaloes were included in the study and divided randomly in 3 groups consisting of healthy control, buffaloes affected with subclinical mastitis with IMI caused by S. aureus and ten buffaloes infected with pathogens other than S. aureus. There was significant increase in levels of Lipid peroxidation (LPO), superoxide dismutase (SOD), catalase (CAT), lactate dehydrogenase (LDH) and Alkaline phosphatase (ALP) while significant reduction in reduced glutathione (GSH) was observed in buffaloes with SCM. No significant difference was observed in blood urea nitrogen (BUN) concentration in buffaloes with SCM. Micromineral estimation revealed higher serum zinc and iron in buffaloes with subclinical mastitis than that of healthy control.