Elicitation of bacoside content using Aspergillus niger filtrate in Bacopa monnieri (Brahmi)

Abstract

Micropropagation technique is used for the elicitation of bacoside content in Bacopa monnieri. In vitro propagation was carried out on Murashige and Skoogs medium supplemented with 6-benzylaminopurine (1.1 µM) and Indole-3- butyric acid (0.30 µM) with 3% sucrose for four weeks. For elicitation variable concentrations of Aspergillus niger filtrate (0, 0.5, 1.0, 1.5, 2.0 ml/L) were incorporated in MS basal medium for 7 days. Present investigation clearly indicated that the Aspergillus niger filtrate significantly influenced the Bacoside production in Brahmi. After seven days elicitation, maximum enhancement in Bacoside (1.62% DW) was reported at lower concentrations of Aspergillus niger filtrate (0.5 ml/L) over control.