Green synthesis, characterization and evaluation of gold nanoparticles using aqueous extract of Ganoderma lucidum

Abstract

The current work describes a reliable green chemistry process for gold nanoparticles synthesis by using Ganoderma lucidum extract. The different characterization techniques used for synthesized nanoparticles were UV-Vis spectroscopy, X-Ray Diffraction, Fourier Transform Infra-Red Spectroscopy (FTIR) and Transmission Electron Microscopy (TEM). The nanoparticles showed purple color with a characteristic SPR crest at 541 nm. Polydispersed nanoparticles irregular in shape and size range from 5 to 30 nm were revealed by TEM. The average crystalline size estimated by the Debye-Scherrer’s method was found to be ~ 10.47 nm. The functional groups responsible for bioreduction are O-H bond in alcohols, phenols, tripertenes and capping agents can be C-H stretching alkyl group and aldehydes, Amide I, O-H Bonding polysaccharides determined by FTIR study. The synthesized nanopartilcles showed potent antibacterial activity against the pathogenic bacteria, as evidenced by their zone of inhibition.