Biochemical studies on electrophoretic banding pattern among the different isolates of Colletotrichum capsici causing fruit rot in chilli

Abstract

Ten isolates of Colletotrichum capsici collected from different regions of Vidarbha and Marathwada (Maharashtra), Bidar district (Karnataka) and Guntur district (Andra Pradesh) showing varied type of pathogenic ability against chilli variety Jayanti and designated as strongly, moderately and weakly pathogenic on the basis of per cent leaf area covered by the disease. Electrophoresis pattern of polyphenol oxidase, esterase and protein have been used to estimate the polymorphism in different fungal populations or species. Therefore, studies were undertaken to know the biochemical variation among the isolates of C. capsici in the form of appearance of isozyme and protein banding pattern on polyacrylamide gel. Electrophoretic protein banding pattern revealed that, Rm values ranged between 0.208 to 0.725 possessing 1 to 4 bands. Isolates among weakly to highly pathogenic, 5 isolates exhibited one band each, while two bands each were existed in 3 isolates. Three bands of Cc6 (Rm value 0.208, 0.350 and 0.725) relates to strongly pathogenic. Polyphenol oxidase isozyme was estimated and Rm values were observed from 0.057 to 0.192, possessing 2 to 3 bands. Eight isolates showed presence of 3 bands each whereas, 2 isolates exhibited 2 bands with Rm values 0.142 and 0.192. Esterase isozyme pattern was observed in all the test isolates of C. capsici and Rm values ranged from 0.048 to 0.544 possessing 3 to 4 bands.