Induced antiproliferative responsive by fraction of Euphorbia umbellata latex inhibits melanoma tumor cells

Abstract

The objective was to evaluate the antitumor potential of the Chloroform (CHCl3) and Acetate (CH3COO) fractions of Euphorbia umbellata latex in melanoma tumor cells (B16-F10) and comparative effect normal fibroblast cells (FN1). The cytotoxicity of the fractions in B16-F10 and normal FN1 cells was evaluated using the MTT colorimetric method, mitochondrial electrical potential (ΔΨm), analysis of organization of cytoskeleton by confocal microscopy and cell cycle phases by flow cytometry. The Acetate fraction obtained greater selectivity for B16-F10 tumor cells, reduced ΔΨm and modified cell morphology, observed by actin filaments. Our results suggest that Acetate and Chloroform can inhibit the proliferation of tumor cells of melanoma B16-F10, promoting apoptosis and inducing a control of proliferation. The Acetate fraction modulating apoptotic pathways and disrupting of organization cytoskeleton.