Effect of different concentrations of ba and naa on callus formation from cotyledonary leaf explants of wild Brinjal (Solanum incanum L.)

Abstract

Different concentrations and combinations of BA and NAA hormones were used in MS medium to observe callus induction from cotyledonary Leaf Explants of Wild Brinjal (Solanum incanum L.) The rate of callus formation varied in different treatments used. Explants were cultured on MS media supplemented with different combinations and BA (0.0 mg/l, 0.5 mg/l, 1.0 mg/l, 1.5 m/l) in combination with NAA (0.2 mg/l, 0.4 mg/l,0.6 mg/l,0.8mg/l) with three replications. BA (1.5mg/l) in combination with NAA (0.2 mg/l) recorded highest result in days for callus initiation, fresh weight of callus and diameter of the callus. The protocol in the study might be useful for the production of disease free and healthy plant materials and also it would be useful for genetic transformation of eggplant using biotechnological approach.