Influence of different varieties, media and storage temperature on pollen viability in mango (Mangifera indica L.)

Abstract

Study was conducted to investigate pollen viability of four mango varieties, viz. Kesar, Alphonso, Mallika and Dudhpendo up to 56 days. Pollen grains were suspended in three different media (n-hexane, paraffin oil and without media) under four different storage temperature (-20 ⁰C, -4 ⁰C, 4 ⁰C and room temperature). Aceto-carmine test was used to determine pollen viability. Variation due to different varieties, media, temperature and their interactions was found significant. Among four varieties, maximum viability was recorded in Kesar during 7, 14, 21, 49 and 56 days of storage, whereas, in Mallika, at 28, 35 and 42 days. In storage media, n-hexane was found best in terms of retaining viability. The storage at -20 ⁰C gave better results in terms of viability in all four varieties. On the basis of results, it could be concluded that pollen grains of Kesar and Mallika could be stored successfully up to 56 days in n-hexane at -20 ⁰C.