Vaccination of Nile Tilapia (Oreochromis niloticus) Using Lipopolysaccharide (LPS) Prepared from Aeromonas hydrophila

Abstract

The efficacy of Aeromonas hydrophila lipopolysaccharide (LPS) as a vaccine was studied in Nile tilapia (Oreochromis niloticus). Vaccine efficacy was measured in terms of the Relative Percent Survival (RPS). The fish was immersed in the vaccine at different duration of exposure. To compare the protective effect of the vaccine, O. niloticus was challenged intraperitoneally with 0.1mL of virulent A. hydrophila suspension at 106cells/.The RPS value of the vaccinated group at 120 sec. immersion was the highest. The results confirm the efficacy of the LPS vaccine prepared from A. hydrophila and showed that the higher the immersion time, the greater was the efficacy of the vaccine.