Testing the effect of silver nanoparticles on the radial growth of R. Solani, Trichoderma Spp. and Pseudomonas fluorescens in vitro

Abstract

In the present investigation, efforts were made to test the effect of nano preparations on the growth of R. Solani, Trichoderma Spp. and Pseudomonas flourescens using poisoned food technique. nano preparations from R. solani, Trichoderma isolate ET-1 five day old culture filtrates, P. fluorescens isolate PF-2 five day old culture filtrate and nano preparations from PDB were assessed along with pure silver nitrate solution at 50%, 5%, 0.5%, 0.05%, 0.005% concentrations of 170 ppm silver nitrate or silver nano preparation. Observations on radial growth were recorded at on 1, 2, and 3 days after inoculation. Studies using poisoned food technique indicated that silver nitrate preparation (pure or bionano) had fungistatic effect on R. solani at lower concentrations (5% and below) as the inhibitory effect was nullified after three days of inoculation. However, at higher concentrations, i. e., at 50% concentration, inhibitory effect continued to exist even after third day. Further, it is also interesting to note that the silver nano preparation from R. solani was inhibitory to itself. The fungistatic effect of silver preparations at