Screening of some new substrate for mass multiplication of Trichoderma harzianum and Pseudomonas fluorescens and determination of suitable moisture level

Abstract

Various low-cost agricultural waste products were screened for mass multiplication of T. harzianum and Pseudomonas fluorescens under normal storage condition and most effective formulated product of T. harzianum and Pseudomonas fluorescens was evaluated in vitro. Among different substrate including some of farm wastes and residues viz. wheat straw, wheat straw + rice husk, rice straw, sugarcane bagasse, sugarcane pressmud, bajra grains + wheat straw, sorghum grains + rice husk, chick pea flour + wheat straw, rotten cow dung + unrotten cow dung. The sorghum grains + rice husk was best growth substrate for T. harzianum and chickpea flour + wheat straw was best substrate for P. flourescens. 70 per cent moisture was appropriate for the growth and mass multiplication of both the antagonist.