Phylogenetic analysis of KRT1.2 gene in Rambouillet sheep

Abstract

The objective of the present study was to investigate the phylogenetic analysis of KRT1.2 gene within the Rambouillet population, maintained at Sheep Breeding and Research Farm, Reasi, Jammu. DNA was isolated by HiPuraTM SPP Blood DNA Kit (HI-MEDIA). An amplicon of 480 bp of KRT1.2 gene was obtained. The coding sequence of the exon is of 347 bp that ranges from 65 bp to 412 bp with 116 amino acids in the coding sequence. A total of 15 nucleotide changes were found. Out of 15 changes 9 were transitions, 5 were transversions and one was multiple changes. Phylogenetic tree of different KRT1.2 gene sequences indicates that sequence KF718797 and KF718804 were the most distant sequences. The highest genetic identity value between different sequences was 99.58 %. The results will help to maintain variability within the population and hence, selection for wool will be more effective.