Effect of subchronic exposure to fluoride and role of Moringa stenopetala on hematologic parameters in mice

Abstract

Any toxicant gaining acess to the body and is not eliminated by liver and kidney, where these organs themselves affected by fluoride accumulation, tend to accumulate and affect the circulatory system. Fluoride accumulated in the blood circulatory system could induce abnormalities generally affecting hematologic parameters. Studies implicated that among the mechanisms by which fluorosis cause damage to tissues is through oxidative stress. Moringa (Moringa stenopetala, Bac.) an endogenous tree to Ethiopia is rich in antioxidants, and has potent antiodxidative effect. to investigate effects of fluorosis on hematologic parameteres, and the role of Moringa stenopetala leaf extract, randomized control study was conducted using 2 months old 24 (12 male and 12 female) Swiss Albino Mice, average weight 33.8±5.5g, randomly allocated in to 5 experimental groups and one control group with free access of pelleted food. The mice took different doses of fluoride, and both fluoride and Moringa stenopetala together for 90 days. On day 91, blood samples were collected by cardiac puncture and complete blood count was measured. There was no significant difference in hematological indices between control mice and those mice treated with fluoride and, fluoride and crude extract of Moringa stenopetala crude extract together (p>0.05). Exposure of this concentration for years or higher concentration for months may increase the probability of fluoride toxicity on hematological parameters.