In vıtro propagatıon of Nepeta nuda Subsp. Lydıae Ph Davıs

Abstract

Due to increased interest in biologically active ingredients, the use of plant sources is increasing day by day. Unconscious harvesting of plants from their natural habitat increases the oppression on endemic plants and biotechnological methods are used to prevent them. The objective of the present work was to develop a simple micropropagation system for Nepeta nuda L. subsp. lydiae PH Davis an endemic plant, thus allowing its exploitation without threat to local biodiversity.Seeds were surface-sterilized and incubated on different in vitro media for germination. Paper bridges and glass beads were used to provide physical support for the seeds. The cultures were maintained at 24 ± 2 o C temperature with a 16-h light period. The results of the experiments have shown that the maximum germination percentage was observed in distilled water (60%). In axillary shoot propagation experiments, the maximum shoot number per explant was obtained on MS medium supplemented with 0.1 mgL-1 Kinetin (5.30 shoot/explant). The highest shoot lenght was obtained on MS medium supplemented with 1 mgL-1 N6-Benzyladenine (7.40). The maximum rooting percentage was obtained on MS medium supplemented with 1 mgL-1 IBA.