Isolation and characterization of chemical constituents from Dalbergia sissoo Roxb. Stem

Abstract

Dalbergia sissoo is an important medicinal plant and commonly known as sisu, shisham, tahli, jag at different parts of world and belongs to family Fabaceae. D. sissoo plant parts contain a large number of chemical constituents; leaves of plant contain trisaccharides, oligosaccharides, phenols and the flower of shisham contains tectorigenin biochanin. Stem-bark contains flavonoids, dalbergichromene cinnamylphenols, 4-phenylchromene. Root-bark contains chalcone, isoflavone biochanin A, flavone, retenoid, dehydroamorphigenin. Methanolic extract of D. sissoo stem was mixed with silica gel (60-120 mesh) and subjected to column chromatography to carry out isolation of the compounds from stem of D. sissoo. Chromatographic separation was carried out over silica gel (60-120 mesh) column and eluted with the solvents of increasing polarity. The column chromatography afforded three compounds suchas D-1(Tritriacontane), D-2 (Dalbergichromene) and D-3 (1,6-Dihydro-1,6-dihydroxy-4-methoxydalbergione).