Lead induced changes in DNA and protein content in Vigna radiata L.

Abstract

Pollution of heavy metals in environment is a growing problem worldwide and currently it has reached at an alarming rate. This heavy metal pollution affects the major threat to Agricultural sector. Lead (Pb) is extensively used in electronic and other industries as an important heavy metal. In the present study phytotoxic effect of heavy metal lead determine on Vigna radiata L. seedling. The different concentrations of lead (25ppm; 50ppm; 75ppm and 100ppm) were used. After the 7 days seedling were harvested and measured the fresh and dry weights, water content, length, chlorophyll content, protein, and changes in DNA content. Further, protein of control and treated seedlings was characterized by SDS-PAGE. Treatment with Pb showed that decrease in dry weight, water content, length and chlorophyll content was observed while DNA and protein concentration increased with Pb concentrations.