In vitro internalization of moldey ion Rhodamine B in ovine mesenchymal stem cell: A comparative study

Abstract

Ovine adipose and bone marrow derived mesenchymal stem cells were isolated by Collagenase digestion and cultured upto passage 6. Cultured adipose and bone marrow derived mesenchymal stem cells (AD-MSCs and BM-MSCs) at passage 4, 5 and 6 were labelled with Moldey Ion Rhodamine-B (MIRB) at the concentration of 25 ul of MIRB in 1ml of culture medium. The internalization of iron particles was assessed by calorimetric technique after 72 hours of incubation. Internalisation did not require any induction method. The results indicated that 16.5 to 18.1 pg and 17.0 to 18.2 pg of Fe was inernalised within the cytoplasm of adipose and bone marrow derived mesenchymal stem cells respectively after 72 hrs of incubation at P4, P5 and P6. However, in both AD-MSCs and BM-MSCs, internalisation with MIRB particles did not affect the viability and proliferation of the stem cells.