Evaluation of Nigella sativa (Black cumin) for anti-cancer and anti-inflammatory activities

Abstract

Natural products obtained from plants are used to treat various diseases in folk medicine including Cancer and inflammation. Nigella sativais annual flowering plants that appertain to Ranunculaceae family. Numerous studies have shown that naturally occurring compounds may support prevention and treatment of various diseases. The aim of our study is to evaluate the cytotoxicity studies for AGS and PANC-1 cell lines and Anti-inflammatory study using RAW264.7 cell line. The N.sativa oil is extracted by soxhelt extraction by using petroleum ether as the whole solvent. For cytotoxicity study the MTT assay is done, which is simple, accurate and yield reproducible result, cytotoxicity is the quality of being toxic to cell. The activity of RAW264.7 cells against iNOS release post LPS stimulation, which is observed calorimetrically. Hence by the graphical studies we can conclude that the crude of N. sativa is consequential for our successive evaluation of Anticancer and Anti-inflammatory modalities.