Isolation and characterization of Endophytic-Rhizospheric phosphate solubilizing bacteria of cumin and their evaluation in vitro

Abstract

The present investigation was carried out during the year 2017-18 and 2018-19 in Laboratory of Microbiology, Devision of crop production, ICAR-National Research Centre for Seed spices Ajmer, Rajasthan (India). The maximum P solubilization zone was 19 mm followed by 17 mm recorded in isolate DCU-251 which was characterized as Pseudomonas aeruginosa (Accession no. MN192165) and DCU-262 (Kosakonia oryzendophytica strain NRCSSDCU262 Accession no. MN192166), respectively. The least solubilization zone was 11 mm due to isolate DCU-551. Phosphate solubilization index ranged 1.6-3.1. Highest SI was 3.1 followed by 2.9 and 2.8 associated with isolate DCU-251(Pseudomonas aeruginosa strain NRCSSDCU251 Accession no. MN192165), DCU-262 (Kosakonia oryzendophytica strain NRCSSDCU262 Accession no. MN192166) and DCU-22 (Bacillus paramycoides strain NRCSSDCU22 Accession no. MN192162), respectively. The Lowest value of SI was 1.5 due to isolate DCU-551. The highest soluble P (326 ݜ‡g ml−1) in pikovskaya’s broth was recorded in bacterial isolate DCU-251(Pseudomonas aeruginosa strain NRCSSDCU251 Accession no. MN192165) and least soluble P was 152 ݜ‡g ml−1 recorded in isolate DCU-553.