In vitro antioxidant, free radical scavenging and DNA binding properties of synthetic metal based imine derivatives of schiff bases

Abstract

In the present study, the antioxidant and DNA binding properties of Schiff base ligand and their Ni(II), Co(II), Cu(II), Zn(II) metal complexes were considered for their possible free radicals scavenging properties associated with various diseases. The different models such as 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferrous ion chelation (FIC), ferric reducing antioxidant power (FRAP), total antioxidant activities (phosphomolybdenum methods) and hydroxyl radical (•OH) radical scavenging activities at different concentrations for antioxidant and DNA absorption spectroscopic analysis were assayed for DNA binding studies. The metal complexes were found to be significant dose-dependent antioxidant activities comparable with that of the classical antioxidants, ascorbic acid, ethylene diaminetetraacetic acid (EDTA) and DNA binding potential with kb 3.487×10-5M-1, 1.858×10-5M-1, 3.090×10-5M-1, 1.367×10-5M-1 and 9.118×10-5M-1 respectively. The compounds exhibited are very reactive towards DPPH radicals, OH radicals and Fe(II) ions and they also actively reduces Fe(III) ion to Fe(II) and Mo(VI) ion to Mo(V) form. The obtained results indicate the importance of Schiff base metal complexes as a source of synthetic antioxidants and anticancer drugs.