The efficacy of Acacia nilotica extract as a disinfectant on addition silicone impressions: An in-vitro study

Abstract

Acacia nilotica is a widely spread plant native to the Nile basin in central and north Sudan. Because of its proven antimicrobial activity it has been extensively used as a traditional and organic substitute for modern medicine. This study aimed to evaluate the efficacy of Acacia nilotica extract as a disinfectant on addition silicone dental impression material and its effect on the dimensional stability of the material. A quasi experimental pre-test post-test design was applied through microbiological testing upon 30 sectional addition silicone impressions. Each sectional impression was immersed in an ethanolic extract of Acacia nilotica with a concentration of 75mg/ml for 10 minutes. The colony forming units (CFU) were recorded before and after the procedure. The dimensional stability was also recorded before and after the procedure using a highly sensitive CAD/CAM machine.Extract of Acacia nilotica showed a reduction in (CFU) from an initial count of 227colonies of Gram positive and Gram negative bacteria to a mean (CFU) of zero when compared to the control group. No statistical difference was found when comparing the mean change of dimensional stability before 6.73± 1.74 and after 6.73±1.71disinfection of addition silicone impressions (p