Influence of different auxin treatment on ex vitro rooting in in vitro regenerated micro shoots of Terminalia arjuna (Arjun)

Abstract

Terminalia arjuna is an important cardio tonic tree, commonly known as Arjun. The realistic aspect of micropropagation technology is its successful execution from lab to field conditions. Rooting of in vitro induced shoot is a critical factor in the micropropagation of woody tree species. The objective of present study was to find out the role of different auxin on ex vitro rooting of micropropagated plantlet of this tree species. For this, in vitro plantlets developed through micropropagation techniques were subjected to different hormonal regime (IBA, NAA & IAA), maximum 62.22% ex vitro rooting was achieved, when plantlets were pulse treated with 200 mg/l (984.25 µM) IBA for 10 min. The rooted plantlets were successfully hardened and acclimatized in poly house then under agro-shade house. These plants were planted in field condition and they showed 60% survival rate in the field conditions.