Effects of graphene quantum dots on physiological characteristics of Caprine oocyst maturation

Abstract

Present study was conducted in department of Veterinary Physiology and Biochemistry at campus of College of Veterinary Science and Animal Husbandry, Anjora, Durg (C.G.). The study was main aim to evaluate the effects of different concentrations of graphene oxide quantum (GQ) dots on oocyte maturation. Epididymal sperms were collected and incubated with different concentrations of GQ dots nanoparticles (0 µg/ml, 10 µg/ml, 50 µg/ml and 100 µg/ml). Caprine oocytes were collected, graded and cultured in vitro in maturation media supplemented with different concentrations of GQ dots nanoparticles (0 µg/ml, 10 µg/ml, 50 µg/ml and 100 µg/ml) and oocytes were observed microscopically and by BCB staining for maturation after 24 hr. Groups IV have least number of matured oocytes & significantly lower number of matured oocytes as compared to control. Increasing concentration of GQ dots has detrimental effects on in vitro maturation of oocytes.