Molecular detection and seroprevalence of foot and mouth disease in goats of Assam, India

Abstract

The present study was undertaken to detect the presence of FMDV in the goats of Assam. LPBE used to derive estimates of location wise incidence of FMDV. In addition, the study also involved the assessment of specific gene targets for detection of PPRV and FMDV by RT-PCR from the clinical samples. To detect the various serotypes of FMD by LPBE 382 serum samples were collected from different groups of goat from 15 districts of Assam. The titre 1.8 was considered to be protective titre in case of vaccinated animals. While in case of infected animals, titre 1.8 may indicate infection by particular type. Out of the total 382 serum samples screened for antibody titer against FMDV serotypes, LPBE titre of 1.8 was observed in 120, 82, 23 serum samples against serotype O, A, and Asia-1 of FMD virus, respectively. The multiplex PCR was used for detection of FMDV serotypes ‘O’, ‘A’ and ‘Asia 1’. Freeze dried internal positive control (Type O: 249 bp; Type A: 376bp; Type Asia 1: 537 bp) were used as a reference of the test. All the samples were found positive for FMD virus serotype ‘O’. Finally to confirm the FMDV RT PCR is done by using O type specific primer which showed prevalence of ‘O’ serotype in Assam.