Green synthesis of Brassica campestris mediated silver nanoparticles, their antibacterial and antioxidant activities

Abstract

Here in the present work silver nanoparticles (AgNPs) were prepared via green method using Brassica campestris plant extract as a reducing as well as stabilizing agents. Synthesis of AgNPs via plant extract is an environmental friendly, simple and single step approach. Nanoparticles formation occurs at room temperature after the addition of plant extract to silver nitrate solution. The obtained AgNPs product was examined by using UV-visible spectroscopy, fourier-transform infrared spectroscopy (FT-IR), powder X-ray powder diffraction (PXRD), thermal gravimetric analysis (TGA), energy dispersive x-ray spectroscopy (EDX) and scanning electron microscope (SEM). SEM images of AgNPs showed that prepared nanoparticles spherical predominantly. EDX showed the elemental nature of AgNPs. XRD confirm the cubic structure of AgNPs, while the FT-IR analysis showed that presence of biomolecules on the nanoparticles surface. Prepared AgNPs showed potent antibacterial activity against water borne pathogens Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) at a minimum inhibitory concentration (MIC) 50 μg/mL. The synthesized AgNPs demonstrate outstanding 1, 1-Diphenyl-2-picrylhydrazyl (DPPH) free radical activity.