Determination of immunoglobulin G (IgG) concentration in buffalo colostrum and serum of new born calves by indirect ELISA

Abstract

The present investigation was carried to measure IgG concentrations in Murrah buffaloes and their respective new born calves. Twenty three newly parturated Murrah buffaloes and their respective new born calves, maintained at Instructional Dairy Farm (IDF), G. B. Pant University of Agriculture and Technology, Pantnagar were used in this study. The indirect ELISA was performed to measure the IgG concentrations in the colostrum (first milk) of dams and serum of their new born calves (6 to 12 hrs post birth). Our results showed that IgG concentration in colostrum of dams and serum of their new born calves were varies from 11 to 122.1 mg/ml with a mean of 56.98 ± 5.71 mg/ml and 4.2 to 14.88 mg/ml with a mean of 11.23 ± 0.70 mg/ml, respectively. The results of present finding concluded that, estimation of IgG level helps in prediction of health status of dams and calf at an early age.