Identification of single nucleotide polymorphism (SNP) of prolactin gene in white leghorn and its association with production traits

Abstract

Marker assisted selection (MAS) is form a genomic selection that can complement the conventional method of selection in poultry breeding. MAS can be done using different molecular markers, one among them is single nucleotide polymorphism (SNP). The main objective of the present study was identification of SNP of prolactin gene in White Leghorn and its association with production traits. A total of 200 birds White Leghorn were randomly selected from AICRP on Poultry Improvement, Mannuthy. Blood samples were collected from the randomly selected birds and isolation of genomic DNA was done by using DNA isolation kit. PCR and PCR-RFLP analysis was carried out to find a SNP at C-2402T site of promoter region of prolactin gene. The genotypes are designated as CC, CT and TT for C-2402T. All the birds were observed with same genotype (CC) and production performance.